In Situ Monitoring of Photodynamic Inactivation of the Membrane Functions of Bacteria Using Electrochemical Sensors

DOI PDF Web Site Web Site Web Site 3 Citations 32 References
  • KATO Hisato
    Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • KOMAGOE Keiko
    Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • INOUE Tsuyoshi
    Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • KATSU Takashi
    Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

Search this article

Abstract

The photodynamic inactivation of the membrane functions of bacteria was analyzed in situ, using K+ and tetraphenylphosphonium (TPP+) electrodes, as well as an oxygen electrode. Tetrakis(4-N-trimethylaminophenyl)porphine (TTMAPP) and rose bengal were used, since both dyes act strongly on bacteria, such as Staphylococcus aureus. After a short time lag, they inhibited the respiration of bacteria and increased the permeability of the cytoplasmic membrane to K+, while dissipating the membrane potential. This combination of sensors is quite useful for visualizing the actions of photosensitizers on the bacterial membrane. TTMAPP and rose bengal impaired the bacterial function by reducing the membrane potential within minutes of photo-irradiation.

Journal

  • Analytical Sciences

    Analytical Sciences 26 (10), 1019-1021, 2010

    The Japan Society for Analytical Chemistry

Citations (3)*help

See more

References(32)*help

See more

Related Projects

See more

Keywords

Report a problem

Back to top