生体膜の機能を探る物性測定－スピンラベル法からイメージング法まで－

• 大木 和夫

  東北大学 大学院理学研究科 物理学専攻

書誌事項

タイトル別名

• Physical Property Measurement Investigating the Function of theBiomembrane–from spin labeling to imaging–
• セイタイマク ノ キノウ オ サグル ブッセイ ソクテイ スピンラベルホウ カラ イメージングホウ マデ

抄録

The spin labeling method has greatly contributed in the research of the biomembrane. Especially, it has beenwidely used in the research of the membrane fluidity. And it gave an important knowledge for the construction of thefluid mosaic biomembrane model proposed by Singer and Nicolson. Extended spin labeling methods, TEMPO andspin–spin exchange, were applied to the studies of phase transition and phase separation, respectively, and manyremarkable results were obtained. In the field of cell biology, introduction of green fluorescence protein (GFP) anddevelopment of confocal laser scanning microscope (CLSM) have elucidated important biological function by CLSMimaging. The author and colleagues have developed microscopic imaging instrument using environment sensitivefluorescence dye, laurdan, and have applied it to study of ‘raft’.

収録刊行物

• 膜

  膜 33 (5), 215-220, 2008

  日本膜学会

キーワード

• spin labeling
• membrane fluidity
• phase separation
• microscopic imaging
• laurdan

詳細情報

• CRID: 1390282681398274816
• NII論文ID: 10026876470
• NII書誌ID: AN0023215X
• DOI: 10.5360/membrane.33.215
• ISSN: 18846440
• ISSN: 03851036
• NDL書誌ID: 9664392
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9664392
• Web Site: https://www.jstage.jst.go.jp/article/membrane/33/5/33_215/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008351147
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可