Meth-A細胞における倍数性の変化に伴うレクチン結合性の変化について

• 佐久真 正弘

  金沢医科大学病院中央臨床検査部

• 藤川 孝三郎

  金沢医科大学総合医学研究所

書誌事項

タイトル別名

• Variations of Lectin Binding with Changes of Ploidy in Meth-A Cells

抄録

The lectin binding that is related to the structure of sugar chain of cell surface is available an indicator to changes for the character of cell. Tetraploid and octaploid Meth-A cells were established from the diploid cells. To examine the lectin binding, diploid, tetraploid and octaploid Meth-A cells growing exponentially were double-stained with propidium iodide and 7 sort of FITC labeled lectins, wheat germ agglutinin (WGA), concanavalin A (Con A), Ricinus communis agglutinin Ⅰ(RCA₁₂₀), peanut agglutinin (PNA), soybean agglutinin (SBA), Ulex europeaus agglutinin Ⅰ(UEA-Ⅰ) and Dolichos bifluorous agglutinin (DBA), and measured for their fluorescence by flow cytometry. 　The extent of lectin binding was different by the lectins used. It was suggested that the expression of sugar chain changes by polyploidization

収録刊行物

• サイトメトリーリサーチ

  サイトメトリーリサーチ 13 (1), 39-44, 2003

  日本サイトメトリー学会

キーワード

• Lectin
• Polyproidization
• Meth-A cells
• glycochain density
• DNA histogram

詳細情報

• CRID: 1390282679448767232
• NII論文ID: 10026899266
• NII書誌ID: AN10356261
• DOI: 10.18947/cytometryresearch.13.1_39
• ISSN: 24240664
• ISSN: 09166920
• Web Site: https://search.jamas.or.jp/link/ui/2004193006
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可