Notes for DNA Measurement of Propidium Iodide Stained Cells with Flow Cytometery

• Oga Atsunori

  Department of Pathology Yamaguchi University School of Medicine

• Murakami Tomoyuki

  Department of Clinical Research National Sanyo Hospital

• Kondo Tomoko

  Department of Pathology Yamaguchi University School of Medicine

• Niina Midori

  Department of Clinical Laboratory National Sanyo Hospital

• Ishiguro Kimio

  Department of Clinical Laboratory Shuto General Hospital

• Sakamoto Keishi

  Department of Clinical Laboratory National Iwakuni Medical Center

• Chochi Yasuyo

  Department of Pathology Yamaguchi University School of Medicine

• Kawauchi Shigeto

  Department of Clinical Research National Sanyo Hospital

• Sasaki Kohsuke

  Department of Pathology Yamaguchi University School of Medicine

Bibliographic Information

Other Title

• Flow Cytometer によるDNA量測定
• －PI染色，DNAヒストグラム解析，coefficient of variationを中心として－

Abstract

Improvement of staining and measuring methods for DNA or cell cycle analyses by flow cytometry (FCM) has been continued by many researchers for years. Japan Cytometry Society (JCS) and the organizations which were past of JCS have borne the central role in Japan. Now, the application range of FCM is greatly expanded and to operate FCM machine became very easy. This paper has been written for the purpose of recognizing again the notes for improving coefficient variation(CV). It is necessary to understand that CV value and DNA histogram may be influenced on various factors. Furthermore, it is important to understand about a possibility of changing the DNA amount of G0/G1 cells or the affinity between double strands DNA and propidium iodide can change, when we analyze DNA histogram.

Journal

• Cytometry Research

  Cytometry Research 14 (2), 15-21, 2004

  Japan Cytometry Society

Keywords

• coefficient of variation
• DNA histogram
• DNA ploidy
• DNA aneuploidy
• cell cycle

Details

• CRID: 1390282679448997376
• NII Article ID: 10026899616
• NII Book ID: AN10356261
• DOI: 10.18947/cytometryresearch.14.2_15
• ISSN: 24240664
• ISSN: 09166920
• Web Site: https://search.jamas.or.jp/link/ui/2005071874
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed