Degree of immobilization of synthetic RGDS(PO_3H_2)PA peptides on titanium surfaces

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Author(s)

    • ABE Yasuhiko
    • Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Hiroshima University Graduate School of Biomedical Sciences
    • OKAZAKI Yohei
    • Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Hiroshima University Graduate School of Biomedical Sciences
    • HIASA Kyou
    • Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Hiroshima University Graduate School of Biomedical Sciences
    • HIRATA Isao
    • Department of Biomaterial Science, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Sciences
    • YOSHIDA Yasuhiro
    • Department of Biomaterials, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
    • TAJI Tsuyoshi
    • Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Hiroshima University Graduate School of Biomedical Sciences
    • SUZUKI Kazuomi
    • Department of Biomaterials, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
    • OKAZAKI Masayuki
    • Department of Biomaterial Science, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Sciences
    • AKAGAWA Yasumasa
    • Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Hiroshima University Graduate School of Biomedical Sciences

Abstract

The purpose of this study was to characterize the chemical interaction between titanium surfaces and the peptide RGDS(PO<sub>3</sub>H<sub>2</sub>)PA (P-RGD) synthesized from RGD peptide (RGD) and <I>o</I>-phospho-L-serine (P-Ser), and to determine the degree of peptide immobilization on the titanium surface. X-ray photoelectron spectroscopy showed that the adsorption amount of RGD was significantly smaller than those of P-Ser and P-RGD (<I>p</I><0.05). Furthermore, although it appeared that P-RGD bonded to the surface, ultrasonic rinsing with water caused it to dissociate, releasing RGD and leaving only S(PO<sub>3</sub>H<sub>2</sub>)PA bonded to the surface. These findings show that although it remains difficult to obtain a stable P-RGD layer, the phosphate functional group greatly improves immobilization of the molecule on titanium surfaces.

Journal

  • Dental Materials Journal

    Dental Materials Journal 29(6), 668-672, 2010-11-01

    The Japanese Society for Dental Materials and Devices

References:  20

Codes

  • NII Article ID (NAID)
    10026962580
  • NII NACSIS-CAT ID (NCID)
    AA10443149
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    02874547
  • Data Source
    CJP  J-STAGE 
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