Measurement of respiratory function using whole-body plethysmography in unanesthetized and unrestrained nonhuman primates

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Author(s)

    • Iizuka Hiromi IIZUKA Hiromi
    • Pharmacology Safety Assessment Group, Nonclinical Research Center, Drug Development Service Segment, Mitsubishi Chemical Medience Co.
    • Sasaki Kazuaki SASAKI Kazuaki
    • Pharmacology Safety Assessment Group, Nonclinical Research Center, Drug Development Service Segment, Mitsubishi Chemical Medience Co.
    • OBO Mayumi
    • Pharmacology Safety Assessment Group, Nonclinical Research Center, Drug Development Service Segment, Mitsubishi Chemical Medience Co.
    • IMAIZUMI Masakazu
    • Pharmacology Safety Assessment Group, Nonclinical Research Center, Drug Development Service Segment, Mitsubishi Chemical Medience Co.
    • ATAI Hiroshi
    • Pharmacology Safety Assessment Group, Nonclinical Research Center, Drug Development Service Segment, Mitsubishi Chemical Medience Co.

Abstract

Extended term, continuous measurement and observation of drug responses were performed to examine the feasibility of a custom-made whole-body plethysmograph for measuring respiratory function in unanesthetized, unrestrained monkeys. Using this apparatus, respiratory function (respiration rate, tidal volume, and minute volume) was observed for 23 hr in unanesthetized, unrestrained cynomolgus monkeys (<i>Macaca fascicularis</i>). The respiration rate, tidal volume, and minute volume in the light period (7:00 to 19:00) reached approximately 30% to 50% higher values than in the dark period (19:00 to 7:00), thus clearly exhibiting circadian variation in the cynomolgus monkey respiratory functions. Administration of morphine (10 mg/kg, s.c.) resulted in sustained reduction in tidal volume and minute volume, and ketamine (30 mg/kg [sub-anesthetic dose], i.m.) also produced sustained reduction in respiration rate, tidal volume, and minute volume.  With dimorpholamine (1 mg/kg, i.v.) or caffeine (10 mg/kg, s.c.), respiration rate, tidal volume, and minute volume increased.  Physiological saline (1 ml/kg, s.c. and 0.1 ml/kg, i.v.) and chlorpromazine (10 mg/kg, s.c.) produced no clear-cut changes in respiration rate, tidal volume, or minute volume. From the above results, we conclude that our custom-made whole-body plethysmograph is useful for measuring respiratory function in unanesthetized and unrestrained monkeys.

Journal

  • The Journal of Toxicological Sciences

    The Journal of Toxicological Sciences 35(6), 863-870, 2010-12-01

    The Japanese Society of Toxicology

References:  17

Codes

  • NII Article ID (NAID)
    10026963114
  • NII NACSIS-CAT ID (NCID)
    AN00002808
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    03881350
  • NDL Article ID
    10931788
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z19-1022
  • Data Source
    CJP  NDL  J-STAGE 
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