Fungal Cellulases of Glycosyl Hydrolase Family 7 Catalyze Lactose Condensation
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- Koizumi Hideyo
- Faculty of Agriculture and Life Science, Hirosaki University
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- Totani Kazuhide
- Department of Chemical Engineering, Ichinoseki National College of Technology
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- Kitamoto Noriyuki
- Food Research Center, Aichi Industrial Technology Institute
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- Sato Shota
- Faculty of Agriculture and Life Science, Hirosaki University
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- Ohmachi Tetsuo
- Faculty of Agriculture and Life Science, Hirosaki University
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- Yoshida Takashi
- Faculty of Agriculture and Life Science, Hirosaki University
Bibliographic Information
- Other Title
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- GHファミリー7に属する糸状菌セルラーゼがラクトースの縮合反応を触媒する
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Abstract
β-1,4-Endoglucanase of glycosyl hydrolase family (GHF) 7 from a fungus Aspergillus oryzae catalyzed the condensation between lactose (Lac) and alkanols. With Lac as a glycosyl donor, methanol, ethanol, 1-butanol and 1-hexanol and 1,6-hexanediol (HD) could be aglycons. The maximal condensation was observed between 250 mM Lac and 1.5 M HD at 30°C, pH 5.0. Transglycosylation to HD occurred to a similar extent with Lac-β-pNP or with cellobiose-β-pNP as the sugar donors, while the condensation between N-acetyllactosamine and HD was negative. Lac-condensation activity was also observed with the cellulase from Fusarium oxysporum, which belongs to GHF7. These suggested that fungal cellulases belonging to GHF7 should possess an ability to perform the condensation using Lac as a glycosyl donor.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 57 (4), 239-243, 2010
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390001206293078016
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- NII Article ID
- 10026967689
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- NII Book ID
- AN10453916
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- ISSN
- 18807291
- 13447882
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- NDL BIB ID
- 10875288
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed