Mechanism for signal transduction in the induced-raft domains as revealed by single-molecule tracking

• Suzuki Kenichi G. N.

  Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science & Technology Agency (JST) Institute for Integrated Cell-Material Sciences (iCeMS), Institute for Frontier Medical Sciences, Kyoto University

• Kusumi Akihiro

  Institute for Integrated Cell-Material Sciences (iCeMS), Institute for Frontier Medical Sciences, Kyoto University Membrane Mechanism Protect, International Cooperative Research Project (ICORP), Japan Science & Technology Agency (JST)

Bibliographic Information

Other Title

• １分子追跡が明らかにした誘導ラフト領域におけるシグナル変換機構
• 1分子追跡が明らかにした誘導ラフト領域におけるシグナル変換機構[含 英語文]
• 1 ブンシ ツイセキ ガ アキラカ ニ シタ ユウドウ ラフト リョウイキ ニ オケル シグナル ヘンカン キコウ ガン エイゴブン

Abstract

Raft domains have been proposed to work as a platform where raftophilic signaling molecules assemble and interact for efficient signal transduction. However, this raft hypothesis has been difficult to prove. Our recent single-molecule tracking experiments revealed that cytoplasmic signaling molecules were frequently, but very transiently recruited to the rafts formed on demand by the clusters of raftophilic glycosylphosphatidylinositol (GPI)-anchored receptors (e.g., CD59) that were generated after the engagement of the receptors by the binding of extracellular signaling molecules. All of the cytoplasmic signaling molecules examined thus far, Gαi2, Lyn, and PLCγ, exhibited short residency times of ~200 ms within the CD59-cluster rafts. This recruitment period of each individual signaling molecule was short, compared with the periods of overall bulk activation of these molecules by a factor of 4,000. Argument has been advanced that the analogue bulk signal, which lasts for over several thousands seconds, is generated by the superposition of the short-lived, digital-like individual signals, which last for a fraction of a second.<br>

Journal

• Trends in Glycoscience and Glycotechnology

  Trends in Glycoscience and Glycotechnology 20 (116), 341-351, 2008

  FCCA(Forum: Carbohydrates Coming of Age)

Keywords

• single-molecule tracking
• plasma membrane
• membrane skeleton
• signal transduction
• induced raft domain

Details

• CRID: 1390001204368950144
• NII Article ID: 10026975802
• NII Book ID: AA10995236
• DOI: 10.4052/tigg.20.341
• ISSN: 18832113
• ISSN: 09157352
• NDL BIB ID: 10160330
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10160330
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed