Immunohistochemical Study of Osteodentin in the Unerupted Rat Incisor

• Hosoya Akihiro

  Department of Oral Histology, Matsumoto Dental University

• Nakamura Hiroaki

  Department of Oral Histology, Matsumoto Dental University

• Akahane Shoji

  Institute for Dental Science, Matsumoto Dental University

• Yoshiba Kunihiko

  Division of Cariology, Department of Oral Health Science, Course for Oral Life Science, Niigata University Graduate School of Medical and Dental Sciences

• Yoshiba Nagako

  Division of Cariology, Department of Oral Health Science, Course for Oral Life Science, Niigata University Graduate School of Medical and Dental Sciences

• Ninomiya Tadashi

  Institute for Dental Science, Matsumoto Dental University

• Hoshi Kazuto

  Department of Menicon Cartilage and Bone Regeneration, Graduate School of Medicine, The University of Tokyo

• Sahara Noriyuki

  Institute for Dental Science, Matsumoto Dental University

• Kasahara Etsuo

  Department of Endodontics and Operative Dentistry, Matsumoto Dental University

• Ozawa Hidehiro

  Institute for Dental Science, Matsumoto Dental University

Abstract

To characterize osteodentin in the pre-functional rat incisor, we performed histological and histochemical evaluation of the anterior apex of the incisor in 3-day-old rats. The unerupted incisor was composed of osteodentin, with numerous cells present in the anterior apex. The osteodentin was immunopositive for osteocalcin, bone sialoprotein, and dentin sialoprotein, with an immunolocalization pattern similar to that of dentin. Back-scattered electron microanalysis (BSE) and electron probe microanalysis (EPMA) showed that osteodentin was not uniformly calcified. These results indicate that osteodentin in the rat incisor possesses dentin-like characteristics, and may be fragile in structure.

Journal

• Journal of Oral Biosciences

  Journal of Oral Biosciences 48 (2), 132-137, 2006

  Japanese Association for Oral Biology

Keywords

• osteodentin
• rat incisor
• dentin sialoprotein
• immunohistochemistry
• back scattered electron microanalysis

Details

• CRID: 1390282680189370880
• NII Article ID: 10027102099
• NII Book ID: AA11896386
• DOI: 10.2330/joralbiosci.48.132
• ISSN: 18803865
• ISSN: 13490079
• Web Site: https://search.jamas.or.jp/link/ui/2007103767
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed