Effects of ion beam irradiation on mutation induction and nuclear DNA content in chrysanthemum

• Yamaguchi Hiroyasu

  Institute of Radiation Breeding, National Institute of Agrobiological Sciences Present address: National Institute of Floricultural Science

• Shimizu Akemi

  Institute of Radiation Breeding, National Institute of Agrobiological Sciences

• Hase Yoshihiro

  Quantum Beam Science Directorate, Japan Atomic Energy Agency

• Tanaka Atsushi

  Quantum Beam Science Directorate, Japan Atomic Energy Agency

• Shikazono Naoya

  Quantum Beam Science Directorate, Japan Atomic Energy Agency

• Degi Konosuke

  Institute of Radiation Breeding, National Institute of Agrobiological Sciences Present address: Miyakojima Branch, Okinawa Prefectural Agricultural Research Center

• Morishita Toshikazu

  Institute of Radiation Breeding, National Institute of Agrobiological Sciences Present address: National Agricultural Research Center for Hokkaido Region (Memuro)

抄録

We investigated the effects of ion beams, in comparison to gamma rays, on mutation induction and nuclear DNA content as an index of radiation damage in Chrysanthemum morifolium. Leaf segments were irradiated with 220 MeV carbon ions (mean linear energy transfer = 107 keV/μm), 320 MeV carbon ions (76 keV/μm), 100 MeV helium ions (9 keV/μm), and gamma rays. The nuclear DNA content was measured and frequency of flower color mutations was investigated in regenerated plants. The number of plants with reduced nuclear DNA content increased with increasing irradiation doses of 320 MeV carbon ions, 100 MeV helium ions, and gamma rays. In contrast, they did not increase with 220 MeV carbon ions, even when the dose was increased. Irradiation treatment with 220 and 320 MeV carbon ions and gamma rays had a similar effect on mutation induction, while the effect of 100 MeV helium ions was not as great. Thus, the effects of irradiation treatments on mutation induction and nuclear DNA content differed according to the type of ion beams. 220 MeV carbon ion beam seemed to be the most appropriate among the three types of ion beams because it gave a high mutation frequency with low damage to chromosomes.<br>

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 60 (4), 398-404, 2010

  日本育種学会

キーワード

• chrysanthemum
• flower color mutation spectrum
• gamma rays
• ion beams
• mutation frequency
• nuclear DNA content

詳細情報

• CRID: 1390001204722833280
• NII論文ID: 130004146249
• NII論文ID: 10027120351
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.60.398
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 10925575
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10925575
• Web Site: http://www.jstage.jst.go.jp/article/jsbbs/60/4/60_4_398/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可