Tsukuba-green : A Fluorescent Dye that Emits Green Fluorescence Useful for Live-cell Imaging
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- SENDA Naoko
- Graduate School of Pure and Applied Sciences, University of Tsukuba
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- MIWA Yoshihiro
- Department of Molecular Pharmacology, Institue of Basic Medical Sciences, University of Tsukuba
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- TANAKA Junko
- Center for Tsukuba Advanced Research Alliance, University of Tsukuba
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- MOMOTAKE Atsuya
- Graduate School of Pure and Applied Sciences, University of Tsukuba
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- ARAI Tatsuo
- Graduate School of Pure and Applied Sciences, University of Tsukuba
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Author(s)
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- SENDA Naoko
- Graduate School of Pure and Applied Sciences, University of Tsukuba
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- MIWA Yoshihiro
- Department of Molecular Pharmacology, Institue of Basic Medical Sciences, University of Tsukuba
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- TANAKA Junko
- Center for Tsukuba Advanced Research Alliance, University of Tsukuba
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- MOMOTAKE Atsuya
- Graduate School of Pure and Applied Sciences, University of Tsukuba
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- ARAI Tatsuo
- Graduate School of Pure and Applied Sciences, University of Tsukuba
Abstract
A new fluorescent dye, 4-ethoxycarbonylmethyl-2-methyl-7-hydroxyquinoline (TG) was obtained unexpectedly from the BiCl<sub>3</sub>-catalyzed Pechmann condensation. TG emits green fluorescence at 509 nm in water. For live-cell imaging applications, TG derivatives TG1–TG3 were prepared. Under physiological conditions, HEp-2 cells were immediately stained upon addition of a solution of TG3.
Journal
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- Chemistry Letters
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Chemistry Letters 39(3), 308-310, 2010-03-05
The Chemical Society of Japan
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