Elution Behavior of Proteins in Capillary Chromatography Using an Untreated Fused-silica Capillary Tube and a Water-Hydrophilic-Hydrophobic Organic Mixture Carrier Solvent

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  • Masuhara Yuji
    Department of Chemical Engineering and Materials Science, Doshisha University
  • Jinno Naoya
    Department of Chemical Engineering and Materials Science, Doshisha University
  • Hashimoto Masahiko
    Department of Chemical Engineering and Materials Science, Doshisha University
  • Tsukagoshi Kazuhiko
    Department of Chemical Engineering and Materials Science, Doshisha University

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We examined the elution behavior of isoluminol isothiocyanate (ILITC)-labeled proteins (alcohol dehydrogenase (ADH) and bovine serum albumin (BSA)) in a capillary chromatography system using an untreated fused-silica capillary tube and a water–acetonitrile–ethyl acetate mixture carrier solution. The mixture of ILITC and ILITC-labeled protein (ADH or BSA) was analyzed using this system with a chemiluminescence detector; the ILITC and the labeled protein were separated in this order with an organic solvent-rich carrier solution, while they were eluted in the reverse order with a water-rich carrier solution. In addition, the mixture of ILITC, ILITC-labeled ADH, and ILITC-labeled BSA was added to this system, and the analytes of the mixture were separated and confirmed with the individual peaks on the chromatograms.

収録刊行物

  • Chemistry Letters

    Chemistry Letters 39 (7), 688-689, 2010

    公益社団法人 日本化学会

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