Simultaneous Determination of Isoniazid, Pyrazinamide, Rifampicin and Acetylisoniazid in Human Plasma by High-Performance Liquid Chromatography

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Author(s)

    • SHEN Mei
    • Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University
    • ZOU Fei
    • Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University

Abstract

A sensitive and accurate high-performance liquid-chromatography method was developed and validated for the simultaneous determination of pyrazinamide (PZA), isoniazid (INH), rifampicin (RFP) and acetylisoniazid (AcINH) in human plasma. Separation was performed on a Max-RP C<sub>12</sub> column using gradient elution and a flow-rate program. The mobile phase was methanol–acetonitrile–buffer (20 mM of heptanesulfonic acid sodium, pH 2.5) with a ratio of 10:8:82 (v/v/v) at the initial phase. All calibration curves had good linearity (<i>r</i><sup>2</sup> > 0.99) between the test ranges. The intra- and inter-day precision was less than 8.8% in good accuracy (<15%). The limit of detection with a signal-to-noise (<i>S/N</i>) of 3 was 0.014, 0.009, 0.023 and 0.054 μg mL<sup>−1</sup> for PZA, AcINH, INH and RFP, respectively. The method was selective, sensitive and reliable, and is a good alternative for routine therapeutic drug monitoring of the four compounds during the treatment of tuberculosis patients.

Journal

  • Analytical Sciences

    Analytical Sciences 26(11), 1133-1138, 2010-11-10

    The Japan Society for Analytical Chemistry

References:  25

Codes

  • NII Article ID (NAID)
    10027315955
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09106340
  • NDL Article ID
    10873074
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z54-F482
  • Data Source
    CJP  NDL  J-STAGE 
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