Effect of Urea on the Ion-association Capillary Electrophoresis Separation of the Anionic Metal Complexes Using Hydrophobic Tetraalkylammonium As Ion-Association Agent Effect of Urea on the Ion-Association Capillary Electrophoresis Separation of the Anionic Metal Complexes Using Hydrophobic Tetraalkylammonium as Ion-Association Agent

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Abstract

The effect of urea as an electrophoretic buffer solution modifier on the ion-association (IA) capillary electrophoresis (CE) separation of four anionic metal complexes of Al(III), Co(III), Cr(III), and Fe(III) with 2,2′-dihydroxyazobenezene-5,5′-disulfonate (DHABS) using a hydrophobic ion-association agent, tetrapentylammonium bromide, was studied. The mutual separation of the four anionic metal-DHABS complexes was not achieved without the addition of urea in the electrophoretic buffer solution. However, the addition of 1.5 M urea in the electrophoretic buffer solution brought about a complete separation of the four metal complexes. The ion-association constants between all metal-DHABS complexes and tetrapentylammonium in an aqueous urea solution were smaller than those in a neat aqueous solution. This indicates the hydrophobic interaction contributing to the ion-association between analytes and ion-association agent during IA-CE separation processes can be controlled with the addition of urea to the electrophoretic buffer solution. Another advantage of adding urea was a substantial enhancement of separation efficiency with a reduction of the half-bandwidth of the peaks. Also, a reduction of the electrophoretic mobility of the electroosmotic flow when urea was added was much less than when organic solvents were used.

Journal

  • Analytical Sciences

    Analytical Sciences 26(11), 1151-1156, 2010-11-10

    The Japan Society for Analytical Chemistry

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Codes

  • NII Article ID (NAID)
    10027316032
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09106340
  • NDL Article ID
    10873128
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z54-F482
  • Data Source
    CJP  NDL  IR  J-STAGE 
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