Initiation, Persistence, and Cessation of the Series of Intracellular Ca2+ Responses during Fertilization of Bovine Eggs.
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- NAKADA Ken
- Department of Physiology, Tokyo Women’s Medical College, Shinjuku-ku, Tokyo 162, Japan
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- MIZUNO Jinji
- Research Laboratory, MIC Co., Ltd. Nasu-machi, Tochigi-ken 329-32, Japan
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- SHIRAISHI Koichi
- Department of Physiology, Tokyo Women’s Medical College, Shinjuku-ku, Tokyo 162, Japan
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- ENDO Kenji
- Research Laboratory, MIC Co., Ltd. Nasu-machi, Tochigi-ken 329-32, Japan
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- MIYAZAKI Shunichi
- Department of Physiology, Tokyo Women’s Medical College, Shinjuku-ku, Tokyo 162, Japan
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- タイトル別名
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- Initiation Persistence and Cessation of
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A long-lasting series of transient rises in intracellular Ca2+ concentration ([Ca2+]i) were recorded with Ca2+ imaging during fertilization of bovine eggs matured in vivo or in vitro. The first Ca2+ response could be recorded in zona pellucida-free eggs within 1 min after sperm-egg contact, when a preceding slow [Ca2+]i rise reached 150-170 nM. The response had the peak of 565 ±32 nM (n=15; maximum 780 nM) and the duration of 4-6 min, being significantly larger and longer than any succeeding response (359 ± 12 nM, n=68; 2 min). The series of Ca2+ transients occurred at constant intervals of 19-20 min without attenuation in the magnitude for more than 9 h. Ca2+ transients became smaller progressively during pronuclear migration and nuclear envelope breakdown, and no response occurred in 2-cell embryo. In unfertilized eggs, injection of inositol 1, 4, 5-trisphophate (InsP3) induced a Ca2+ transient and the sulfhydryl reagent thimerosal caused repetitive Ca2+ transients. These responses were markedly inhibited or blocked by preinjection of a monoclonal antibody to the mouse InsP3 receptor. However, sperm-induced responses were not blocked in 2/3 of eggs examined. Another mechanism may be involved in addition to InsP3-induced Ca2+ release from intracelluar stores.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 41 (1), 77-84, 1995
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681309809408
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- NII論文ID
- 10027316648
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- NII書誌ID
- AA10936678
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- COI
- 1:CAS:528:DyaK2MXnt1CgsLY%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 3618324
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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