24時間室温保存したマウス卵母細胞子からの産仔の作出

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献11件 参考文献64件
  • WAKAYAMA Sayaka
    Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe Department of Life Science, Graduate School of Science and Technology, Kobe University
  • THUAN Nguyen Van
    Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
  • KISHIGAMI Satoshi
    Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
  • OHTA Hiroshi
    Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
  • MIZUTANI Eiji
    Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe Graduate School of Agricultural Science, Tohoku University
  • HIKICHI Takafusa
    Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
  • MIYAKE Masashi
    Department of Life Science, Graduate School of Science and Technology, Kobe University
  • WAKAYAMA Teruhiko
    Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe

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タイトル別名
  • Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

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To determine the feasibility of preserving oocytes without freezing, we stored mouse oocytes in several media at different temperatures for one day. Confocal microscopy of the metaphase-II spindle in these stored oocytes revealed gross abnormalities in both the spindle and the arrangement of chromosomes. The abnormal spindles could not be rescued by transplanting the aged spindle-chromosome complex into a fresh enucleated oocyte. A diploid parthenogenetic development showed that some of the oocytes stored at room temperature could still develop into blastocysts (10-57%). However, oocytes stored in a refrigerator (5%) or incubator (0%) lost the potential almost entirely. Fertilization of room-temperature-preserved oocytes with fresh spermatozoa by ICSI or IVF resulted in, respectively, 4 and 10%, full-term births. These results suggest that when oocytes are stored at room temperature for one day, most have irreversible damage not only to their cytoplasm but also to the spindle. However, since at least a few percent of stored oocytes retained the potential for full-term development, it may be possible to overcome these problems and develop a simple method for preserving mammalian oocytes without freezing.<br>

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