Cytogenetic Analysis and Developmental Assessment of Mouse Embryos Derived from In Vitro Fertilization of Oocytes Reconstructed by Meiosis-II Chromosome Transplantation
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- MITSUI Akinori
- Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
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- YOSHIZAWA Midori
- Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
Bibliographic Information
- Other Title
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- Cytogenetic analysis and developmental assessment of mouse embryos derived from in vitro fertilization of oocytes reconstructed by meiosis-2 chromosome transplantation
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Abstract
An electrofusion methodology for transferring meiosis-II chromosomes (M-II-t) has not been completely established. The present study compared the use of two temperatures (fusion at 37 C for Group A and 25 C for Group B) during an electrofusion procedure for mouse oocyte M-II-t and investigated the cytogenetic normality and developmental competence of embryos derived from in vitro fertilization using oocytes reconstructed by M-II-t. The M-II-t oocytes were fertilized in vitro and cultured to the blastocyst stage; the resultant embryos were analyzed cytogenetically. Subsequently, chromosomal normality of the resultant embryos at the prometaphase stage of first cleavage division and the integrity of the meiosis-II spindles of the reconstructed oocytes were analyzed. The success rate of electrofusion in Group B was 92.1%, which was significantly different from that in Group A (49.2%) (P<0.05). The fertilization rates (A, 80.7%; B, 77.2%) and development rates (A, 70.9%; B, 65.5%) in the M-II-t groups were significantly lower than those in the control group (95.0 and 92.2%, respectively) (P<0.05). The incidence of chromosomal abnormalities in the Group A embryos (20.5%) at the blastocyst stage was significantly higher than that in the control group embryos (8.5%) (P<0.05), but the incidence of chromosomal abnormalities in Group B (12.5%) was not significantly different compared with the other groups. A temperature of 25 C during the electrofusion procedure for M-II-t resulted in a good fusion rate, good development rate, and efficient production of chromosomally normal blastocysts. Furthermore, the incidence of chromosomal abnormalities in the first cleavage embryos at the prometaphase stage in Group B (9.6%) did not differ significantly from that in the control group (6.6%). The spindle morphology of the M-II-t oocytes in Group B was normal.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 53 (2), 357-366, 2007
The Society for Reproduction and Development
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Keywords
Details 詳細情報について
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- CRID
- 1390001206335255040
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- NII Article ID
- 130000053912
- 10027328215
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 8717223
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed