Mouse exo utero development system: Protocol and troubleshooting
Bibliographic Information
- Other Title
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- Mouse <i>exo utero</i> development system: Protocol and troubleshooting
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Abstract
ABSTRACT The exo utero development system allows us to manipulate or operate on live embryos of mice or rats at mid- to late gestation stages, from late organogenetic to histogenetic periods, and keep them alive in situ until the analysis of their effects at a desired time point. We can examine the effects of injecting bioactive molecules or cells into targeted parts of a live embryo, destroying specific embryonic regions, or performing fetal surgery. This system is far simpler and more time- and cost-effective for in vivo functional analyses than establishing genetically modified mouse lines and provides a fine-tuned experiemental design for developmental scientists. To promote use of the mouse exo utero development system, we elaborate on the technical procedures, discuss critical points for troubleshooting the system, and illustrate some apparatuses essential for fetal microinjection.
Journal
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- official journal of Congeital Anomalies Research Association of Japan
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official journal of Congeital Anomalies Research Association of Japan 48 (4), 183-187, 2008
The Japanese Teratology Society
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Keywords
Details 詳細情報について
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- CRID
- 1390007536268227456
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- NII Article ID
- 10027387768
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- NII Book ID
- AN10066760
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- ISSN
- 24331503
- 17414520
- 00372285
- 09143505
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed