ダイオキシン計測のための表面プラズモンイムノセンサー用感応膜の試作と評価  [in Japanese] Selectivity and Regeneratability Evaluation of Antigen Analogous-Modified Surface Film for Polychlorinated Dibenzodioxin Immunosensor Using Surface Plasmon Resonance Measurement  [in Japanese]

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Author(s)

    • 安澤 幹人 YASUZAWA Mikito
    • 徳島大学大学院ソシオテクノサイエンス研究部 Department of Chemical Science and Technology, Institute of Technology and Science, The University of Tokushima
    • 二神 規通 FUTAGAMI Norimichi
    • 徳島大学大学院先端技術科学教育部 Department of Chemical Science and Technology, Graduate School of Advanced Technology and Science, The University of Tokushima
    • 渕脇 雄介 [他] FUCHIWAKI Yusuke
    • 徳島大学大学院先端技術科学教育部 Department of Chemical Science and Technology, Graduate School of Advanced Technology and Science, The University of Tokushima
    • 薮谷 智規 YABUTANI Tomoki
    • 徳島大学大学院ソシオテクノサイエンス研究部 Department of Chemical Science and Technology, Institute of Technology and Science, The University of Tokushima
    • 本仲 純子 MOTONAKA Junko
    • 徳島大学大学院ソシオテクノサイエンス研究部 Department of Chemical Science and Technology, Institute of Technology and Science, The University of Tokushima

Abstract

For the development of a sensitive and accurate immunosensor, the production of a highly specific surface to the corresponding antibody is essential. Moreover, a specific surface having good reusability is worthwhile for constructing a prompt auto-monitoring immunosensing system. In this study, antigen analogous-modified gold substrates having a specific adsorption property to an anti-dioxin antibody, were prepared by the immobilization of 2,4,5-trichlorophenoxy (TCP) derivatives on a gold surface using various methods. An anti-dioxin antibody, which has a good correlation immunoaffinity with the total toxic equivalent quantity (TEQ) of polychlorinated dibenzo-<i>p</i>-dioxins, was employed as a specific antibody, and the specification of the surface was evaluated by a comparison of the surface plasmon resonance (SPR) responses to a specific anti-dioxin antibody and non-specific anti-hemoglobin antibody. In order to obtain a firm and stable immobilization of TCP, a carboxyl group was introduced on gold by a self-assemble monolayer (SAM) technique, and TCP derivatives were attached to the SAM surface with covalent bonding directly and indirectly through the presence of a hexamethylene chain, bovine serum albumin (BSA), and ethylene glycol oligomer (EGO). A TCP derivative-immobilized substrate, prepared using BSA, showed poor reusability. A TCP derivative-immobilized substrate having EGO was highly specific to an anti-dioxin antibody, while that prepared by the direct immobilization of TCP and with introduction of a hexamethylene chain presented nonspecific adsorption. In addition, a TCP derivative-immobilized substrate having EGO showed a good reproducible response from the second repetition, and was reusable more than seven times.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 60(1), 81-86, 2011-01-05

    The Japan Society for Analytical Chemistry

References:  21

Codes

  • NII Article ID (NAID)
    10027410005
  • NII NACSIS-CAT ID (NCID)
    AN00222633
  • Text Lang
    JPN
  • Article Type
    NOT
  • ISSN
    05251931
  • NDL Article ID
    10972446
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z17-9
  • Data Source
    CJP  NDL  J-STAGE 
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