高速原子間力顕微鏡によるタンパク質の動態撮影  [in Japanese] Video Imaging of Protein Molecules in Action by High-speed Atomic Force Microscopy  [in Japanese]

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Author(s)

    • 安藤 敏夫 ANDO Toshio
    • 金沢大学理工研究域数物科学系 Department of Physics, College of Science and Engineering, Kanazawa University

Abstract

Proteins are dynamic in nature and work at the single-molecule level. Reflecting this fact, single-molecule fluorescence microscopy has been widely exploited to understand how proteins operate. However, what we can observe thereby is the dynamic behaviour of individual fluorescent spots (each being emitted from a fluorophore attached to a selected locus of the molecule), not of the protein molecules themselves. The structure of proteins has been studied by electron microscopy, X-ray crystallography and NMR, but the obtained structures are essentially static. This long-standing problem prevailing throughout biological research has been recently overcome by the development of high-speed atomic force microscopy that enables simultaneous recording of the structure and dynamics of functioning biomolecules with high spatiotemporal resolution.<br>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 51(1), 22-25, 2011-01-25

    The Biophysical Society of Japan General Incorporated Association

References:  19

Codes

  • NII Article ID (NAID)
    10027414748
  • NII NACSIS-CAT ID (NCID)
    AN00129693
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    05824052
  • NDL Article ID
    10995404
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z18-49
  • Data Source
    CJP  NDL  IR  J-STAGE 
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