Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells

• Shimizu Yuri

  Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Fujishiro Hitomi

  Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Matsumoto Kanako

  Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Sumi Daigo

  Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Satoh Masahiko

  Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University

• Himeno Seiichiro

  Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

Bibliographic Information

Other Title

• Toxicogenomics/proteomics report: Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells

Abstract

To investigate the effects of chronic exposure to arsenite on the gene expression profiles of mast cells, microarray analysis was performed on rat basophilic leukemia RBL-2H3 cells exposed to arsenite for 28 days. Upregulated genes include calcium-binding S100 proteins such as S100A9, S100A10, S100A6, and S100A13, and granzymes B and C. Among S100 proteins, S100A9 showed the highest expression (8.62-fold of untreated cells) after 4-weeks of exposure to arsenite. As S100A8 and S100A9 comprise a heterodimer called calprotectin, and are implicated in the development of atherosclerosis and cancer, mRNA levels of both S100A8 and S100A9 were analyzed. The results demonstrated that exposure of RBL-2H3 cells to arsenite for a few weeks induces marked increases in mRNA levels of S100A8 and S100A9.

Journal

• The Journal of Toxicological Sciences

  The Journal of Toxicological Sciences 36 (1), 135-139, 2011

  The Japanese Society of Toxicology

Keywords

• Arsenic
• Mast cell
• S100A8
• S100A9

Details

• CRID: 1390282679878369664
• NII Article ID: 10027420605
• NII Book ID: AN00002808
• DOI: 10.2131/jts.36.135
• ISSN: 18803989
• ISSN: 03881350
• NDL BIB ID: 10999498
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10999498
• Web Site: http://www.jstage.jst.go.jp/article/jts/36/1/36_1_135/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011323242
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed