Restraint Stress Induces Connexin-43 Translocation via α-Adrenoceptors in Rat Heart

Access this Article

Search this Article

Author(s)

    • UNUMA Kana
    • Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo
    • YAHAGI Naoki
    • Department of Emergency and Critical Care Medicine, University of Tokyo Hospital
    • UEYAMA Takashi
    • Departments of Anatomy and Cell Biology, Wakayama Medical University
    • YOSHIDA Ken-ichi
    • Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo

Abstract

<b><i>Background:</i></b> Immobilization (IMO) confers emotional stress in animals and humans. It was recently reported that IMO in rats induced translocation of connexin-43 (Cx43) to gap junctions (GJs) and attenuated arrhythmogenesis with GJ inhibition, and Cx43 translocation in the ischemic heart was also shown. Few reports show the contribution of adrenoceptors to Cx43 upregulation in cardiomyocytes, but the involvement of adrenoceptors and ischemia in Cx43 translocation in IMO remains elusive. <b><i>Methods and Results:</i></b> Male Sprague-Dawley rats underwent IMO and the ventricular distribution of Cx43 was examined by western blotting. IMO induced translocation of Cx43 to the GJ-enriched membrane fraction, with a peak at 60min. The IMO-induced Cx43 translocation was inhibited by pretreatment with the α<sub>1</sub>-adrenoceptor blockers, prazosin (1mg/kg, PO) and bunazosin (4mg/kg, PO), but not with either the β<sub>1</sub>-blocker, metoprolol (10mg/kg, IP), or the β<sub>1+2</sub>-blocker, propranolol (1mg/kg, PO). The translocation was inhibited by the nitric oxide, donor isosorbide dinitrate (100μg·kg<sup>-1</sup>·min<sup>-1</sup>, IV), possibly through sympathetic inhibition. Hypoxia inducible factor-1α was not redistributed by IMO. The β-blockers, but not the α-blockers, inhibited the premature ventricular contractions (PVCs) induced by IMO. <b><i>Conclusions:</i></b> Translocation of Cx43 to the GJ-enriched fraction occurs via the α<sub>1</sub>-adrenoceptor pathway, independently of ischemia. The β-adrenoceptor pathway contributes to the inducing of PVCs in IMO. (<i>Circ J</i> 2010; <b>74:</b> 2693-2701)<br>

Journal

  • Circulation Journal

    Circulation Journal 74(12), 2693-2701, 2010-11-25

    The Japanese Circulation Society

References:  44

Cited by:  1

Codes

  • NII Article ID (NAID)
    10027424654
  • NII NACSIS-CAT ID (NCID)
    AA11591968
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13469843
  • Data Source
    CJP  CJPref  J-STAGE 
Page Top