Timing-controlled Decompaction of Polyplexes In Vivo Greatly Enhances Transgene Expression

Yoichi Tachibana, Tomoko Hashimoto, Hisae Nozaki, Akira Murakami, Tetsuji Yamaoka

doi:10.1246/cl.2010.1238

Timing-controlled Decompaction of Polyplexes In Vivo Greatly Enhances Transgene Expression

DOI PDF 参考文献27件

Yoichi Tachibana

Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute
Tomoko Hashimoto

Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute
Hisae Nozaki

Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute
Akira Murakami

Department of Biomolecular Engineering, Kyoto Institute of Technology
Tetsuji Yamaoka

Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute

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<jats:title>Abstract</jats:title> <jats:p>Compaction extent of polyplexes was successfully regulated by cold treatment in buffer solution using thermoresponsive gene carriers composed of linear poly(ethyleneimine) (l-PEI) and alkyl side chains. Plasmid DNA (pCMV-Luc) was transfected to COS-1 cells using these carriers with different cold treatments. The luciferase expression was greatly enhanced when cells were treated at 4 °C in well-defined timing. This was a direct observation of how intracellular destabilization in regulated timing is important for nonviral gene transfection.</jats:p>