A Sandwich ELISA for the Determination of Beef Meat Content in Processed Foods

• KOTOURA Satoshi

  Marudai Food Co., Ltd

• MURAKAMI-YAMAGUCHI Yukie

  Kyoto Women's University, Department of Food and Nutrition

• NAKAMURA Miyuki

  Marudai Food Co., Ltd

• MIAKE Kiyotaka

  Marudai Food Co., Ltd

• SUGIYAMA Masaaki

  Marudai Food Co., Ltd

• TANABE Soichi

  Hiroshima University, Graduate School of Biosphere Science

• NARITA Hiroshi

  Kyoto Women's University, Department of Food and Nutrition

抄録

Two kinds of polyclonal antibodies against beef myoglobin were produced by immunizing rabbits with denatured myoglobin or with a peptide having the amino acid sequence unique to beef myoglobin. The latter peptide antibody reacted specifically with beef myoglobin without cross-reacting with other food proteins or with pork and chicken myoglobin. A sandwich ELISA, in which this peptide antibody was used as a capture antibody and the anti-denatured myoglobin antibody as an enzyme-labeled antibody, was also specific against beef myoglobin. When the beef content was determined in pork-or chicken-based model foods by this sandwich ELISA, the recoveries of beef protein agreed well with the actual mixing ratio of beef. Moreover, it could be confirmed by this method that the labeling of beef usage in several commercially processed foods was reasonable. Thus, a sandwich ELISA suitable for the evaluation of beef content in various foods was established.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 15 (6), 613-618, 2009

  公益社団法人 日本食品科学工学会

キーワード

• beef myoglobin
• peptide antibody
• sandwich ELISA
• food allergy
• meat deception

詳細情報

• CRID: 1390282679435699200
• NII論文ID: 10027495651
• NII書誌ID: AA11320122
• DOI: 10.3136/fstr.15.613
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可