Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1

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Abstract

<I>Rhodococcus</I> sp. RHA1 induces two biphenyl dioxygenases, the BphA and EtbA/EbdA dioxygenases, during growth on biphenyl. Their subunit genes were expressed in <I>R. erythropolis</I> IAM1399 to investigate the involvement of each subunit gene in their activity and their substrate preferences. The recombinant expressing <I>ebdA1A2A3etbA4</I> and that expressing <I>bphA1A2A3A4</I> exhibited 4-chlorobiphenyl (4-CB) transformation activity, suggesting that these gene sets are responsible for the EtbA/EbdA and BphA dioxygenases respectively. When <I>bphA4</I> and <I>etbA4</I> were swapped to construct the recombinants expressing <I>ebdA1A2A3bphA4</I> and <I>bphA1A2A3etbA4</I> respectively, compatibility between BphA4 and EtbA4 was suggested by their 4-CB transformation activities. When <I>bphA3</I> and <I>ebdA3</I> were swapped, incompatibility between BphA3 and EbdA3 was suggested. BphA and EtbA/EbdA dioxygenases exhibited the highest transformation activity toward biphenyl and naphthalene respectively, and also attacked dibenzofuran and dibenzo-<I>p</I>-dioxin. The wide substrate preference of EtbA/EbdA dioxygenase suggested that it plays a more important role in polychlorinated biphenyl (PCB) degradation than does BphA dioxygenase.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 71(4), 993-1002, 2007-04-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  29

Cited by:  4

Codes

  • NII Article ID (NAID)
    10027513713
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168451
  • NDL Article ID
    8715080
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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