Cloning and Expression of a Novel Sulfotransferase with Unique Substrate Specificity from Bombyx mori
-
- HATTORI Kenji
- Kyoritsu University of Pharmacy
-
- HIRAYAMA Mio
- Kyoritsu University of Pharmacy
-
- SUZUKI Hiroko
- Kyoritsu University of Pharmacy
-
- HAMAMOTO Hiroshi
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
-
- SEKIMIZU Kazuhisa
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
-
- TAMURA Hiro-omi
- Kyoritsu University of Pharmacy
Bibliographic Information
- Other Title
-
- Cloning and Expression of a Novel Sulfotransferase with Unique Substrate Specificity from<i>Bombyx mori</i>
Search this article
Abstract
We identified a cDNA encoding a putative cytosolic sulfotransferase (SULT) by searching the expressed sequence tag database of Bombyx mori, and subsequently obtained the full-length cDNA for this gene via rapid amplification of cDNA ends (RACE). We designated this gene bmST1, and showed by sequence analysis that it belongs to a novel SULT family. The tissue specificity of bmST1 mRNA expression was examined in fifth instar larvae by reverse transcriptase-polymerase chain reaction (RT-PCR), and transcripts were detectable in the silk gland, gut, fat body, and Malpighian tube. A recombinant form of bmST1 was then expressed using a gluthathione S-transferase (GST) gene fusion system, and it was purified from Escherichia coli. Purified bmST1 did not exhibit sulfating activity toward SULT substrates such as 4-nitrophenol, vanillin, hydroxysteroids, or monoamines. Surprisingly, however, recombinant bmST1 showed considerable activity toward 4-nitrocatechol and also gallate esters, although the catechins are not sulfated by this enzyme.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 71 (4), 1044-1051, 2007
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Details 詳細情報について
-
- CRID
- 1390001206480390144
-
- NII Article ID
- 10027513902
-
- NII Book ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 8715304
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed