Inhibitory Effect of Fulvic Acid Extracted from Canadian Sphagnum Peat on Chemical Mediator Release by RBL-2H3 and KU812 Cells

• YAMADA Parida

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• ISODA Hiroko

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• HAN Jun Kyu

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• TALORETE Terence P. N.

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• YAMAGUCHI Tatsuaki

  Laboratory of Organic Resources, Chiba Institute of Technology

• ABE Yukuo

  Graduate School of Life and Environmental Sciences, University of Tsukuba

抄録

Fulvic acid (FA) was extracted and purified from Canadian Sphagnum peat (CP-FA) and characterized by using an element analysis meter, Fourier transform infrared (FT-IR) spectroscopy, electron spin resonance (ESR) spectroscopy, and ¹³C-nuclear magnetic resonance (¹³C-NMR) spectroscopy. To investigate the antiallergic effect of CP-FA, we incubated rat basophilic leukemia (RBL-2H3) cells with 0.001–10.0 μg/ml of CP-FA and determined the β-hexosaminidase release inhibition at different response stages. The intracellular calcium [Ca²⁺]_i level was also determined by using Fluo 3-AM, a calcium-specific fluorescent probe, and the cytotoxicity of CP-FA was determined by the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The results revealed that RBL-2H3 cells incubated for 48 h with 0.001–10.0 μg/ml of CP-FA did not show any decreased viability. CP-FA inhibited the β-hexosaminidase release by IgE-sensitized, antigen-stimulated RBL-2H3 cells at the antigen-antibody binding stage and the antibody-receptor binding stage. CP-FA also inhibited histamine release from A23187 plus PMA- or compound 48/80-stimulated KU812 cells. Furthermore, there was a decrease in the intracellular [Ca²⁺]_i level in IgE-sensitized cells incubated with CP-FA and stimulated with antigen. Our results show that CP-FA may be useful for the treatment or prevention of allergic diseases.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 71 (5), 1294-1305, 2007

  公益社団法人 日本農芸化学会

キーワード

• antiallergy
• β-hexosaminidase
• fulvic acid
• peat
• rat basophilic leukemia (RBL-2H3) cells

詳細情報

• CRID: 1390282681456040064
• NII論文ID: 10027514903
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60702
• COI: 1:STN:280:DC%2BD2szgs1yltw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 8745694
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8745694
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60702
• Web Site: https://search.jamas.or.jp/link/ui/2007330635
• 本文言語コード: en
• データソース種別:
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