The positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis in ethanol production using xylose-fermenting recombinant Saccharomyce cerevisiae

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  • WATANABE Seiya
    Faculty of Engineering, Kyoto University Institute of Advanced Energy, Kyoto University CREST, JST (Japan Science and Technology Agency)
  • PACK Seung Pil
    Institute of Advanced Energy, Kyoto University CREST, JST (Japan Science and Technology Agency)
  • SALEH Ahmed Abu
    Institute of Advanced Energy, Kyoto University
  • ANNALURU Narayana
    Institute of Advanced Energy, Kyoto University
  • KODAKI Tsutomu
    Institute of Advanced Energy, Kyoto University CREST, JST (Japan Science and Technology Agency)
  • MAKINO Keisuke
    Institute of Advanced Energy, Kyoto University CREST, JST (Japan Science and Technology Agency) International Innovation Center, Kyoto University

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タイトル別名
  • The Positive Effect of the Decreased NADPH-Preferring Activity of Xylose Reductase from Pichia stipitis on Ethanol Production Using Xylose-Fermenting Recombinant Saccharomyces cerevisiae
  • The Positive Effect of the Decreased NADPH-Preferring Activity of Xylose Reductase from<i>Pichia stipitis</i>on Ethanol Production Using Xylose-Fermenting Recombinant<i>Saccharomyces cerevisiae</i>
  • Positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae

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We focused on the effects of a mutation of xylose reductase from Pichia stipitis (PsXR) on xylose-to-ethanol fermentation using recombinant Saccharomyces cerevisiae transformed with PsXR and PsXDH (xylitol dehydrogenase from P. stipitis) genes. Based on inherent NADH-preferring XR and several site-directed mutagenetic studies using other aldo-keto reductase enzymes, we designed several single PsXR mutants. K270R showing decreased NADPH-preferring activity without a change in NADH-preferring activity was found to be a potent mutant. Strain Y-K270R transformed with K270R PsXR and wild-type PsXDH showed a 31% decrease in unfavorable xylitol excretion with 5.1% increased ethanol production as compared to the control in the fermentation of 15 g l−1 xylose and 5 g l−1 glucose.

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