Cloning and Functional Expression of Cycloartenol Synthases from Mangrove Species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce

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Author(s)

Abstract

To obtain cDNAs encoding oxidosqualene cyclase (OSC), we cloned two cDNAs, <I>KcCAS</I> and <I>RsCAS</I>, from roots of <I>Kandelia candel</I> (L.) Druce and leaves of <I>Rhizophora stylosa</I> Griff. by homology based PCR method respectively. The deduced amino acid sequences of both OSCs showed 82% homology to cycloartenol synthases from <I>Lotus japonicus</I> (OSC5) and <I>Ricinus cummunis</I> (RcCAS), suggesting that these are cycloartenol synthases of <I>K. candel</I> and <I>R. stylosa</I>. The genes obtained were expressed in a lanosterol synthase deficient <I>Saccharomyces cerevisiae</I> (ERG7) strain, GIL77. GC–MS analysis identified the accumulated reaction product in the yeast transformant to be cycloartenol, indicating that both <I>KcCAS</I> and <I>RsCAS</I> encode cycloartenol synthase.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 71(7), 1788-1792, 2007-07-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  28

Codes

  • NII Article ID (NAID)
    10027517017
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    09168451
  • NDL Article ID
    8840276
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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