A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
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- KOJIMA Katsura
- National Institute of Agrobiological Sciences
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- KUWANA Yoshihiko
- National Institute of Agrobiological Sciences
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- SEZUTSU Hideki
- National Institute of Agrobiological Sciences
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- KOBAYASHI Isao
- National Institute of Agrobiological Sciences
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- UCHINO Keiro
- National Institute of Agrobiological Sciences
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- TAMURA Toshiki
- National Institute of Agrobiological Sciences
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- TAMADA Yasushi
- National Institute of Agrobiological Sciences
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We constructed a new plasmid vector for the production of a modified silk fibroin heavy chain protein (H-chain) in the transgenic silkworm. The plasmid (pHC-null) contained the promoter and the 3′ region of a gene encoding the H-chain and the coding regions for the N-terminal domain and the C-terminal domain of the H-chain. For the model protein, we cloned a foreign gene that encoded EGFP between the N-terminal domain and the C-terminal domain in pHC-null and generated transgenic silkworms that produced a modified H-chain, HC-EGFP. Transgenic silkworms produced HC-EGFP in the posterior part of silk gland cells, secreted it into the lumen of the gland, and produced a cocoon with HC-EGFP as part of the fibroin proteins. N-terminal sequencing of HC-EGFP localized the signal sequence cleavage site to between positions A(21) and N(22). These results indicate that our new plasmid successfully produced the modified H-chain in a transgenic silkworm.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (12), 2943-2951, 2007
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681454157056
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- NII論文ID
- 10027521906
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 9326567
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可