Identification of a Fatty Acyl-CoA Synthetase Gene, lcf2+, Which Affects Viability after Entry into the Stationary Phase in Schizosaccharomyces pombe
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- FUJITA Yasuyuki
- Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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- MITA Satoka
- Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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- OHTSUKA Hokuto
- Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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- AIBA Hirofumi
- Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
Bibliographic Information
- Other Title
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- Identification of a Fatty Acyl-CoA Synthetase Gene,<i>lcf2</i><sup>+</sup>, Which Affects Viability after Entry into the Stationary Phase in<i>Schizosaccharomyces pombe</i>
- Identification of a fatty acyl-CoA synthetase gene, lcf2+, which affect viability after entry into the stationary phase in Schizosaccharomycespombe
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Abstract
The lcf1+ gene, which encodes a long chain fatty acyl-CoA synthetase, is necessary for the maintenance of viability after entry into the stationary phase in Schizosaccharomyces pombe. In this study, we analyzed a paralogous gene, SPBP4H10.11c (named lcf2+), and we present evidence that the gene encodes a new fatty acyl-CoA synthetase. The enzyme preferentially recognized myristic acid as a substrate. A Δlcf2 mutant showed increased viability after entry into the stationary phase in SD medium. A Δlcf1Δlcf2 double mutant showed a severe decrease in long-chain fatty acyl-CoA synthetase activity and a rapid loss of viability after entry into the stationary phase. These results suggest that fatty acid utilization and/or metabolism is important to determine viability in the stationary phase.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (12), 3041-3047, 2007
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001206478069504
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- NII Article ID
- 10027522284
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 9327062
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed