Monitoring Global Gene Expression of Proteases and Improvement of Human Lysozyme Production in the<i>nptB</i>Gene Disruptant of<i>Aspergillus oryzae</i>

• KIMURA Shinya

  Department of Biotechnology, The University of Tokyo

• MARUYAMA Jun-ichi

  Department of Biotechnology, The University of Tokyo

• TAKEUCHI Michio

  Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

• KITAMOTO Katsuhiko

  Department of Biotechnology, The University of Tokyo

書誌事項

タイトル別名

• Monitoring Global Gene Expression of Proteases and Improvement of Human Lysozyme Production in the nptB Gene Disruptant of Aspergillus oryzae

抄録

Aspergillus oryzae has numerous protease genes that might cause proteolytic degradation of heterologously-produced proteins. The productivity of the heterologous protein can be improved by protease gene disruption, but it is difficult to select disruption targets efficiently. In this study, we monitored the expression of 132 protease genes by DNA microarray. A group of protease genes up-regulated during cultivation was identified by clustering analysis. In this protease group, the nptB gene encoding neutral protease II was included as well as the alpA, tppA, and pepA genes, disruption of which has improved human lysozyme (HLY) production. The nptB gene was disrupted to investigate its involvement in HLY production, and nptB disruptants showed an improvement in the production. These observations suggest that monitoring the expression of protease genes is an efficient strategy in screening potential disruption targets for heterologous protein production in A. oryzae.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (2), 499-505, 2008

  公益社団法人 日本農芸化学会

キーワード

• <I>Aspergillus oryzae</I>
• protease gene expression
• DNA microarray
• heterologous protein production
• neutral protease II

詳細情報

• CRID: 1390001206480096640
• NII論文ID: 10027524510
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70582
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 9410241
• Web Site: http://id.ndl.go.jp/bib/9410241
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9410241
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70582
• Web Site: https://search.jamas.or.jp/link/ui/2008216584
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可