Salt-Adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-Glycosidase Activities by Salt-Adaptation

• KIMURA Yoshinobu

  Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• WATANABE Takao

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• KIMURA Mariko

  Faculty of Food Culture, Department of Food System, Kurashiki Sakuyo University

• MAEDA Megumi

  Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University

• MURATA Yoshiyuki

  Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University

• FUJIYAMA Kazuhito

  International Center for Biotechnology, Osaka University

Bibliographic Information

Other Title

• Salt-Adaptation of Tobacco BY2 Cells Induces Change in Glycoform of<i>N</i>-Glycans: Enhancement of Exo- and Endo-Glycosidase Activities by Salt-Adaptation

Abstract

In this report, we describe that a salt adaptation of plant cells induces glycoform changes in N-glycoproteins. Intracellular and cell-wall glycopeptides were prepared from glycoproteins expressed in wild-type BY2 cells and salt-adapted cells. N-Glycans were liberated from those glycopeptides by hydrazinolysis, and the released oligosaccharides were N-acetylated and pyridylaminated. The structures of pyridylaminated (PA-) N-glycans were analyzed by a combination of two-dimensional sugar-chain mapping, MS analysis, and exoglycosidase digestion. In both wild-type cells and salt-adapted cells, the plant complex type structure was predominant among N-glycans expressed on glycoproteins, but we found that the Man2Xyl1Fuc1GlcNAc2 structure was significantly expressed on intracellular and cell-wall glycoproteins of the salt-adapted cells. Furthermore, enhancement of the specific activities of α-mannosidase and β-N-acetylglucosaminidase was observed in the salt-adapted BY2 cells, suggesting that the glycoform changes are due to changes in glycosidase activities.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 72 (2), 514-522, 2008

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• salt-adaptation
• plant glycoprotein
• <I>N</I>-glycan
• glycoform change
• tobacco BY2 cells

Details

• CRID: 1390001206480083968
• NII Article ID: 10027524562
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70592
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 9410260
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9410260
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70592
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed