Isolation and Characterization of<i>Acidithiobacillus ferrooxidans</i>Strain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile

• SUGIO Tsuyoshi

  Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

• WAKABAYASHI Masanori

  Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

• KANAO Tadayoshi

  Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

• TAKEUCHI Fumiaki

  Environmental Management and Safety Section, Health and Environment Center, Okayama University

書誌事項

タイトル別名

• Isolation and Characterization of Acidithiobacillus ferrooxidans Strain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile

抄録

Acidithiobacillus ferrooxidans strain D3-2, which has a high copper bioleaching activity, was isolated from a low-grade sulfide ore dump in Chile. The amounts of Cu²⁺ solubilized from 1% chalcopyrite (CuFeS₂) concentrate medium (pH 2.5) by A. ferrooxidans strains D3-2, D3-6, and ATCC 23270 and 33020 were 1360, 1080, 650, and 600 mg·l⁻¹·30 d⁻¹. The iron oxidase activities of D3-2, D3-6, and ATCC 23270 were 11.7, 13.2, and 27.9 μl O₂ uptake·mg protein⁻¹·min⁻¹. In contrast, the sulfite oxidase activities of strains D3-2, D3-6, and ATCC 23270 were 5.8, 2.9, and 1.0 μl O₂ uptake·mg protein⁻¹·min⁻¹. Both of cell growth and Cu-bioleaching activity of strains D3-6 and ATCC 23270, but not, of D3-2, in the chalcopyrite concentrate medium were completely inhibited in the presence of 5 mM sodium bisulfite. The sulfite oxidase of strain D3-2 was much more resistant to sulfite ion than that of strain ATCC 23270. Since sulfite ion is a highly toxic intermediate produced during sulfur oxidation that strongly inhibits iron oxidase activity, these results confirm that strain D3-2, with a unique sulfite resistant-sulfite oxidase, was able to solubilize more copper from chalcopyrite than strain ATCC 23270, with a sulfite-sensitive sulfite oxidase.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (4), 998-1004, 2008

  公益社団法人 日本農芸化学会

キーワード

• <I>Acidithiobacillus ferrooxidans</I>
• copper bioleaching
• sulfur oxidation
• iron oxidation
• sulfite oxidation

詳細情報

• CRID: 1390282681454417536
• NII論文ID: 10027526575
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70743
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 9481158
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9481158
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70743
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
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