Efficient and Direct Fermentation of Starch to Ethanol by Sake Yeast Strains Displaying Fungal Glucoamylases

• KOTAKA Atsushi

  Research Institute, Gekkeikan Sake Co., Ltd.

• SAHARA Hiroshi

  Research Institute, Gekkeikan Sake Co., Ltd.

• HATA Yoji

  Research Institute, Gekkeikan Sake Co., Ltd.

• ABE Yasuhisa

  Research Institute, Gekkeikan Sake Co., Ltd.

• KONDO Akihiko

  Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University

• KATO-MURAI Michiko

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• KURODA Kouichi

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• UEDA Mitsuyoshi

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

抄録

Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (5), 1376-1379, 2008

  公益社団法人 日本農芸化学会

キーワード

• sake yeast
• <I>Aspergillus oryzae</I>
• glucoamylase
• cell-surface engineering
• bioethanol

詳細情報

• CRID: 1390001206478726912
• NII論文ID: 10027528204
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70825
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 9518956
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9518956
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70825
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可