Reduction of Hg²⁺with Reduced Mammalian Cytochrome<i>c</i>by Cytochrome<i>c</i>Oxidase Purified from a Mercury-Resistant<i>Acidithiobacillus ferrooxidans</i>Strain, MON-1

• SUGIO Tsuyoshi

  Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

• FUJII Mitsuko

  Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

• NINOMIYA Yumika

  Department of Biological Function, Faculty of Agriculture, Okayama University

• KANAO Tadayoshi

  Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

• NEGISHI Atsunori

  Technical Research Institute, Hazama Corporation

• TAKEUCHI Fumiaki

  Environmental Management Center, Okayama University

書誌事項

タイトル別名

• Reduction of Hg2+ with Reduced Mammalian Cytochrome c by Cytochrome c Oxidase Purified from a Mercury-Resistant Acidithiobacillus ferrooxidans Strain, MON-1

抄録

Acidithiobacillus ferrooxidans AP19-3, ATCC 23270, and MON-1 are mercury-sensitive, moderately mercury-resistant, and highly mercury-resistant strains respectively. It is known that 2,3,5,6-tetramethyl-p-phenylendiamine (TMPD) and reduced cytochrome c are used as electron donors specific for cytochrome c oxidase. Resting cells of strain MON-1 had TMPD oxidase activity and volatilized metal mercury with TMPD as an electron donor. Cytochrome c oxidase purified from strain MON-1 reduced mercuric ions to metalic mercury with reduced mammalian cytochrome c as well as TMPD. These mercury volatilization activities with reduced cytochrome c and TMPD were completely inhibited by 1 mM NaCN. These results indicate that cytochrome c oxidase is involved in mercury reduction in A. ferrooxidans cells. The cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 were completely inhibited by 1 μM and 5 μM of mercuric chloride respectively. In contrast, the activity of strain MON-1 was inhibited 33% by 5 μM, and 70% by 10 μM of mercuric chloride, suggesting that the levels of mercury resistance in A. ferrooxidans strains correspond well with the levels of mercury resistance of cytochrome c oxidase.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (7), 1756-1763, 2008

  公益社団法人 日本農芸化学会

キーワード

• <I>Acidithiobacillus ferrooxidans</I>
• cytochrome <I>c</I> oxidase
• mercury resistance
• mercury reduction
• iron oxidase

詳細情報

• CRID: 1390001206480455168
• NII論文ID: 10027529703
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.80070
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 9595739
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9595739
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80070
• 本文言語コード: en
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