Manipulation of Major Membrane Lipid Synthesis and Its Effects on Sporulation in Saccharomyces cerevisiae

• DENG Lan

  Department of Biotechnology, The University of Tokyo

• NAGASAWA Jumpei

  Department of Biotechnology, The University of Tokyo

• ONO Yusuke

  Department of Biotechnology, The University of Tokyo

• ISHIKAWA Yasuhisa

  Department of Biotechnology, The University of Tokyo

• KAKIHARA Toru

  Department of Biotechnology, The University of Tokyo

• FUKUDA Ryouichi

  Department of Biotechnology, The University of Tokyo

• OHTA Akinori

  Department of Biotechnology, The University of Tokyo

Bibliographic Information

Other Title

• Manipulation of Major Membrane Lipid Synthesis and Its Effects on Sporulation in<i>Saccharomyces cerevisiae</i>

Abstract

During the sporulation process of Saccharomyces cerevisiae, meiotic progression is accompanied by de novo formation of the prospore membrane inside the cell. However, it remains to be determined whether certain species of lipids are required for spore formation in yeast. In this study, we analyzed the requirement of the synthesis of phosphatidylethanolamine (PE), phosphatidylcholine (PC), and ergosterol for spore formation using strains in which the synthesis of these lipids can be controlled. When synthesis of PE and PC was repressed, sporulation efficiency decreased. This suggests that synthesis of these phospholipids is vital to proper sporulation. In addition, sporulation was also impaired in cells with a lowered sterol content, raising the possibility that sterol content is also important for spore formation.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 72 (9), 2362-2368, 2008

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• <I>Saccharomyces cerevisiae</I>
• sporulation
• phosphatidylethanolamine
• phosphatidylcholine
• sterol

Details

• CRID: 1390282681456007424
• NII Article ID: 10027532009
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.80265
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 9749265
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9749265
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80265
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed