Manipulation of Major Membrane Lipid Synthesis and Its Effects on Sporulation in Saccharomyces cerevisiae
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- Deng Lan DENG Lan
- Department of Biotechnology, The University of Tokyo
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- Nagasawa Jumpei NAGASAWA Jumpei
- Department of Biotechnology, The University of Tokyo
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- Ono Yusuke [他] ONO Yusuke
- Department of Biotechnology, The University of Tokyo
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- ISHIKAWA Yasuhisa
- Department of Biotechnology, The University of Tokyo
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- KAKIHARA Toru
- Department of Biotechnology, The University of Tokyo
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- FUKUDA Ryouichi
- Department of Biotechnology, The University of Tokyo
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- OHTA Akinori
- Department of Biotechnology, The University of Tokyo
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Author(s)
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- Deng Lan DENG Lan
- Department of Biotechnology, The University of Tokyo
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- Nagasawa Jumpei NAGASAWA Jumpei
- Department of Biotechnology, The University of Tokyo
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- Ono Yusuke [他] ONO Yusuke
- Department of Biotechnology, The University of Tokyo
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- ISHIKAWA Yasuhisa
- Department of Biotechnology, The University of Tokyo
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- KAKIHARA Toru
- Department of Biotechnology, The University of Tokyo
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- FUKUDA Ryouichi
- Department of Biotechnology, The University of Tokyo
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- OHTA Akinori
- Department of Biotechnology, The University of Tokyo
Abstract
During the sporulation process of <I>Saccharomyces cerevisiae</I>, meiotic progression is accompanied by <I>de novo</I> formation of the prospore membrane inside the cell. However, it remains to be determined whether certain species of lipids are required for spore formation in yeast. In this study, we analyzed the requirement of the synthesis of phosphatidylethanolamine (PE), phosphatidylcholine (PC), and ergosterol for spore formation using strains in which the synthesis of these lipids can be controlled. When synthesis of PE and PC was repressed, sporulation efficiency decreased. This suggests that synthesis of these phospholipids is vital to proper sporulation. In addition, sporulation was also impaired in cells with a lowered sterol content, raising the possibility that sterol content is also important for spore formation.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 72(9), 2362-2368, 2008-09-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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