Stereoselective Reduction of Carbonyl Compounds with Actinomycete : Purification and Characterization of Three α-Keto Ester Reductases from Streptomyces avermitilis

Access this Article

Search this Article

Author(s)

Abstract

We achieved the purification of three α-keto ester reductases (<I>Streptomyces avermitilis</I> keto ester reductase, SAKERs-I, -II, and -III) from <I>Streptomyces avermitilis</I> NBRC14893 whole cells. The molecular masses of the native SAKERs-I, -II, and -III were estimated to be 72, 38, and 36 kDa, respectively, by gel filtration chromatography. The subunit molecular masses of SAKERs-I, -II, and -III were also estimated to be 32, 32, and 34 kDa, respectively, by SDS-polyacrylamide gel electrophoresis. The purified SAKERs-II and -III showed a reducing activity for α-keto esters (in particular, for ethyl pyruvate). SAKER-I showed a high reducing activity not only toward the α- and β-keto esters, but also toward α-keto acid. The <small>N</small>-terminal region amino acid sequences of SAKERs-I, -II, and -III were identical to that of a putative oxidoreductase, SAV2750, a putative oxidoreductase, SAV1849, and a putative oxidoreductase, SAV4117, respectively, hypothetical proteins coded on the <I>S. avermitilis</I> genome.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 72(12), 3249-3257, 2008-12-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  33

Codes

  • NII Article ID (NAID)
    10027535299
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    9756100
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
Page Top