Purification and Molecular Cloning of Xylanases from the Wood-Feeding Termite,<i>Coptotermes formosanus</i>Shiraki

• ARAKAWA Gaku

  Center of Molecular Biosciences (COMB), University of the Ryukyus Graduate School of Engineering and Science, University of the Ryukyus

• WATANABE Hirofumi

  National Institute of Agrobiological Sciences

• YAMASAKI Hideo

  Graduate School of Engineering and Science, University of the Ryukyus

• MAEKAWA Hideaki

  Center of Molecular Biosciences (COMB), University of the Ryukyus Graduate School of Engineering and Science, University of the Ryukyus

• TOKUDA Gaku

  Center of Molecular Biosciences (COMB), University of the Ryukyus

書誌事項

タイトル別名

• Purification and Molecular Cloning of Xylanases from the Wood-Feeding Termite, Coptotermes formosanus Shiraki
• Purification and molecular cloning of xylanases from the wood-feeding termites, Coptotermes formosanus Shiraki

抄録

Coptotermes formosanus is one of the most destructive termites in the southern part of Japan as well as in the United States. Hemicellulose is a noncellulosic polysaccharide found in plant cell walls, and xylan is the major constituent of hemicellulose. Since hemicellulose prevents access of cellulolytic enzymes to cellulose, enzymatic hydrolysis of hemicellulose is beneficial for cellulose digestion. We purified three functional xylanases to homogeneity from C. formosanus for the first time. Elution profiles from the whole termite extract suggest that these three xylanases play major roles in xylan digestion in the gut of the termites. The corresponding cDNAs were successfully cloned based on the N-terminal amino acid sequences, encoding GHF11 xylanases. Reverse transcription-PCR using manipulated protozoan cells in the hindgut revealed that the corresponding genes were expressed in the symbiotic flagellate Holomastigotoides mirabile. These results suggest that the GHF11 xylanases that are produced by the symbiotic flagellates play a primary role in xylan degradation in C. formosanus.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (3), 710-718, 2009

  公益社団法人 日本農芸化学会

キーワード

• termite
• xylan
• xylanase
• protist
• GHF11

詳細情報

• CRID: 1390282681456640896
• NII論文ID: 10027539061
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.80788
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10199276
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10199276
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80788
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可