Construction of Flocculent Kluyveromyces marxianus Strains Suitable for High-Temperature Ethanol Fermentation

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Author(s)

Abstract

Flocculating yeasts are highly useful in fermentation processes because these cells can be separated easily from the fermentation mash. However, native yeasts are usually non-flocculating, including <I>Kluyveromyces marxianus</I>, which exhibits a potent high-temperature ethanol fermentation ability. We describe here the construction of flocculent <I>K. marxianus</I> strains <I>via</I> the introduction of the <I>FLO1</I>, <I>FLO5</I>, <I>FLO9</I>, and <I>FLO10</I> genes from <I>Saccharomyces cerevisiae</I>. The <I>S. cerevisiae</I> <I>FLO</I> genes were overexpressed by upstream insertion of the constitutive <I>TDH3</I> promoter, resulting in flocculent <I>S. cerevisiae</I> strains. These <I>TDH3p-FLO</I> sequences were then amplified by PCR and introduced directly into a <I>K. marxianus</I> strain. These <I>K. marxianus</I> strains showed a flocculation phenotype, indicating that the introduced <I>S. cerevisiae</I> <I>TDH3</I> promoter and all <I>FLO</I> genes were functional in this strain. Moreover, a flocculating <I>K. marxianus</I> strain showed the same ethanol production profile as that of its wild-type parent. The <I>K. marxianus</I> flocculating strains we generated should be useful in the future development of cost-effective fed-batch and continuous fermentation systems at high temperatures.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 73(5), 1090-1095, 2009-05-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  39

Cited by:  1

Codes

  • NII Article ID (NAID)
    10027540950
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168451
  • NDL Article ID
    10234834
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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