Effects of Ca[2+] on refolding of the recombinant hemolytic lectin CEL-3

• HISAMATSU Keigo

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• UNNO Hideaki

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• GODA Shuichiro

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• HATAKEYAMA Tomomitsu

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

書誌事項

タイトル別名

• Effects of Ca2+ on Refolding of the Recombinant Hemolytic Lectin CEL-III
• Effects of Ca²⁺on Refolding of the Recombinant Hemolytic Lectin CEL-III

抄録

CEL-III is a hemolytic lectin isolated from Cucumaria echinata. Although recombinant CEL-III (rCEL-III) expressed in Escherichia coli showed very weak hemolytic activity compared with native protein, it was considerably enhanced by refolding in the presence of Ca²⁺. This suggests that Ca²⁺ supported correct folding of the carbohydrate-binding domains of rCEL-III, leading to effective binding to the cell surface and subsequent self-oligomerization.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (5), 1203-1205, 2009

  公益社団法人 日本農芸化学会

キーワード

• lectin
• hemolysis
• refolding
• calcium
• carbohydrate-binding

詳細情報

• CRID: 1390001206476990976
• NII論文ID: 10027541543
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.80793
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10235284
• Web Site: http://id.ndl.go.jp/bib/10235284
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10235284
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80793
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可