Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) Using Erwinia herbicola Cells Carrying a Mutant Transcriptional Regulator TyrR

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  • KOYANAGI Takashi
    Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University Graduate School of Biostudies, Kyoto University
  • KATAYAMA Takane
    Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
  • SUZUKI Hideyuki
    Graduate School of Biostudies, Kyoto University Graduate School of Science and Technology, Kyoto Institute of Technology
  • ONISHI Akiko
    Graduate School of Biostudies, Kyoto University
  • YOKOZEKI Kenzo
    AminoScience Laboratories, Ajinomoto Co., Inc. Graduate School of Agriculture, Kyoto University
  • KUMAGAI Hidehiko
    Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

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  • Hyperproduction of 3,4-Dihydroxyphenyl-<scp>L</scp>-alanine (<scp>L</scp>-Dopa) Using<i>Erwinia herbicola</i>Cells Carrying a Mutant Transcriptional Regulator TyrR

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Abstract

In the last few decades, enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-dopa) using tyrosine phenol-lyase (Tpl) has been industrialized. This method has an intrinsic problem of tyrosine contamination because Tpl is synthesized under tyrosine-induced conditions. Herein, we constructed a hyper-L-dopa-producing strain by exploiting a mutant TyrR, an activator of tpl. The highest productivity was obtained for the strain grown under non-induced conditions. It was 30-fold higher than that obtained for tyrosine-induced wild-type cells.

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