Ferrous Iron Production Mediated by Tetrathionate Hydrolase in Tetrathionate-, Sulfur-, and Iron-Grown<i>Acidithiobacillus ferrooxidans</i>ATCC 23270 Cells

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  • SUGIO Tsuyoshi
    Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
  • TAHA Taher M.
    Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
  • TAKEUCHI Fumiaki
    Environmental Management Center, Okayama University

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  • Ferrous Iron Production Mediated by Tetrathionate Hydrolase in Tetrathionate-, Sulfur-, and Iron-Grown Acidithiobacillus ferrooxidans ATCC 23270 Cells

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When tetrathionate-grown Acidithiobacillus ferrooxidans ATCC 23270 cells were incubated with ferric ions and tetrathionate at pH 3.0, ferrous ions were produced enzymatically. Fe3+-reductase, which catalyzes Fe3+ reduction with tetrathionate, was purified to homogeneity not only from tetrathionate-grown, but also from sulfur- and iron-grown A. ferrooxidans ATCC 23270 cells. The results for apparent molecular weight measured by SDS–PAGE (52.3 kD) and the N-terminal amino acid sequences of the purified enzymes from iron-, sulfur, and tetrathionate-grown cells (AVAVPMDSTG) indicate that Fe3+-reductase corresponds to tetrathionate hydrolase. The evidence that tetrathionate-grown A. ferrooxidans ATCC 23270 cells have high iron-oxidizing activity at the early log phase, comparable to that of iron-grown ATCC 23270 cells, is supported by our finding that tetrathionate hydrolase produces Fe2+ from tetrathionate during growth on tetrathionate. This is the first report on ferric reductase activity associated with tetrathionate hydrolase.

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