Stable Expression of the Chlorocatechol Dioxygenase Gene from Ralstonia eutropha NH9 in Hybrid Poplar Cells

• OHMIYA Yasunori

  Forest Tree Breeding Center

• ONO Toshiro

  Graduate School of Bioresources, Mie University

• TANIGUCHI Toru

  Forest Tree Breeding Center

• ITAHANA Naoei

  Forest Tree Breeding Center

• OGAWA Naoto

  Faculty of Agriculture, Shizuoka University

• MIYASHITA Kiyotaka

  National Institute for Agro-Environmental Sciences

• OHMIYA Kunio

  Graduate School of Bioresources, Mie University

• SAKKA Kazuo

  Graduate School of Bioresources, Mie University

• KIMURA Tetsuya

  Graduate School of Bioresources, Mie University

Bibliographic Information

Other Title

• Stable Expression of the Chlorocatechol Dioxygenase Gene from<i>Ralstonia eutropha</i>NH9 in Hybrid Poplar Cells

Abstract

The cbnA gene encoding chlorocatechol dioxygenase from the soil bacterium Ralstonia eutropha NH9 under the control of a modified cauliflower mosaic virus 35S promoter was introduced into a hybrid poplar (Populus tremula × P. tremuloides). Integration of the cbnA gene in transgenic poplar was confirmed by PCR and genomic Southern blot analysis. Expression of the cbnA gene was analyzed by Western blot analysis. Transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muconate.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 73 (6), 1425-1428, 2009

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• chlorocatechol
• poplar
• <I>Ralstonia eutropha</I>
• transgenic

Details

• CRID: 1390282681455577600
• NII Article ID: 10027542830
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.80848
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 10267569
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10267569
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80848
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed