Production of human monoclonal antibodies against FcεRIα by a method combining in vitro immunization with phage display

• TOMIMATSU Kosuke

  Graduate School of Systems Life Sciences, Faculty of Agriculture, Kyushu University

• MATSUMOTO Shin-ei

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• YAMASHITA Makiko

  Graduate School of Systems Life Sciences, Faculty of Agriculture, Kyushu University

• TERUYA Kiichiro

  Graduate School of Systems Life Sciences, Faculty of Agriculture, Kyushu University Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• KATAKURA Yoshinori

  Graduate School of Systems Life Sciences, Faculty of Agriculture, Kyushu University Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• KABAYAMA Shigeru

  Nihon Trim Co., Ltd.

• SHIRAHATA Sanetaka

  Graduate School of Systems Life Sciences, Faculty of Agriculture, Kyushu University Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

書誌事項

タイトル別名

• Production of Human Monoclonal Antibodies against Fc.EPSILON.RI.ALPHA. by a Method Combining in Vitro Immunization with Phage Display
• Production of human monoclonal antibodies against Fc イプシロン RI アルファ by a method combining in vitro immunization with phage display
• Production of Human Monoclonal Antibodies against FcεRIα by a Method Combining<i>in Vitro</i>Immunization with Phage Display

抄録

An in vitro immunization protocol using human peripheral blood mononuclear cells (PBMC) was developed to generate human antigen-specific antibodies. Monoclonal antibodies have great potential, and in particular, efficient acquirement of monoclonal antibodies against membrane proteins provides advantages. In this study, we tried to generate a human monoclonal antibody against the high affinity IgE receptor, FcεRIα, using a method combining in vitro immunization and phage display. Heavy and light chain variable region genes were obtained from PBMC immunized in vitro with FcεRIα-expressed KU812F cells. Subsequently a combined phage antibody library 6×10³ in the size was generated. Antigen-specific phage antibody clones were selected by panning with recombinant FcεRIα and recombined to produce human IgG format antibodies using CHO cells. The antibodies exhibited specific binding against FcεRIα. These results suggest that one can obtain membrane protein-specific human monoclonal antibodies from a relatively small phage antibody library using in vitro immunized PBMCs.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (7), 1465-1469, 2009

  公益社団法人 日本農芸化学会

キーワード

• IgE receptor
• <I>in vitro</I> immunization
• human monoclonal antibody
• phage display

詳細情報

• CRID: 1390001206478665088
• NII論文ID: 10027543021
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.80640
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10303134
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10303134
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80640
• Web Site: https://search.jamas.or.jp/link/ui/2009346154
• 本文言語コード: en
• データソース種別:
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