Establishment of a Primary Culture Method for Mouse Intestinal Epithelial Cells by Organ Culture of Fetal Small Intestine

DOI Web Site Web Site 2 Citations 83 References
  • YAMADA Kiyoshi
    Department of Applied Biological Chemistry, The University of Tokyo
  • SATO Kanako
    Department of Applied Biological Chemistry, The University of Tokyo
  • MORISHITA Satoru
    Department of Applied Biological Chemistry, The University of Tokyo
  • KAMINOGAWA Shuichi
    Department of Applied Biological Chemistry, The University of Tokyo
  • TOTSUKA Mamoru
    Department of Applied Biological Chemistry, The University of Tokyo

Search this article

Abstract

Studies of the physiological functions of intestinal epithelial cells (IECs) have been limited by the difficulty of primary culture of IEC. We established a method for primary culture of mouse IEC by culturing fragments of fetal small intestines pretreated with EDTA. This method reproducibly resulted in the expansion of cytokeratin-positive epithelial cells, and vigorous expansion of the epithelial cells was observed only from intestinal fragments of embryonic days 15-16. These cells expressed alkaline phosphatase activity and major histocompatibility complex (MHC) class II molecules, indicating the mature phenotype of IEC in a small intestine. The cells also presented antigens to CD4+ T cells. Furthermore, the cells expressed various cytokines and chemokines, and the expression was enhanced by bacterial stimulation. These results indicate that the primary-cultured mouse IEC prepared by the method established here can be a beneficial tool in study of the functions of IECs, especially in mucosal immunity.

Journal

Citations (2)*help

See more

References(83)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top