Mustard Oil in "Shibori Daikon" a Variety of Japanese Radish, Selectively Inhibits the Proliferation of H-ras-Transformed 3Y1 Cells

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  • YAMASAKI Masao
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
  • OMI Yusuke
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
  • FUJII Naoko
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
  • OZAKI Asako
    Nutrition College, Osaka City Institute of Public Health and Environmental Sciences
  • NAKAMA Akihiko
    Nutrition College, Osaka City Institute of Public Health and Environmental Sciences
  • SAKAKIBARA Yoichi
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
  • SUIKO Masahito
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
  • NISHIYAMA Kazuo
    Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

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  • Mustard Oil in “<i>Shibori Daikon</i>” a Variety of Japanese Radish, Selectively Inhibits the Proliferation of H-<i>ras</i>-Transformed 3Y1 Cells

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Abstract

Cruciferous vegetables and their isothiocyanates are promising foods and agents for cancer prevention. We focus here on the effects of mustard oil (SMO) in a variety of the Japanese radish, Shibori Daikon (Raphanus sativus), on the proliferation of 3Y1 rat fibroblasts and the H-ras-transformed derivative, HR-3Y1-2. SMO (1.6 μg/ml) inhibited the proliferation of HR-3Y1-2, but not 3Y1 after 24 h after treatment. A cell cycle analysis showed that SMO induced G2/M arrest after 6 h, although this effect was not observed 24 h after the treatment. SMO transiently decreased the cellular reduced glutathione level accompanied with up-regulation of the intracellular reactive oxygen species 2–3 h post-treatment. Glutathione ethyl ester and N-acetyl-L-cysteine prevented the growth inhibitory effect of SMO. This mustard oil extract consisted of 95.6% 4-methylthio-3-butenyl isothiocyanate and 4.4% 4-methylthiobutyl isothiocyanate. SMO selectively inhibited H-ras-transformed 3Y1 cells associated with transient oxidative stress via reduced glutathione (GSH) depletion.

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