Cloning and Functional Analysis of an Allene Oxide Synthase in Physcomitrella patens

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  • P.K.G.S.S. BANDARA
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • TAKAHASHI Kosaku
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • SATO Michio
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • MATSUURA Hideyuki
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • NABETA Kensuke
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University

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  • Cloning and Functional Analysis of an Allene Oxide Synthase in<i>Physcomitrella patens</i>

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Abstract

Jasmonic acid (JA) is a plant hormone that plays important roles in a large number of processes in stress adaptation and development in flowering plants. A search of genome database indicated the existence of allene oxide synthase (AOS), an enzyme of JA biosynthesis, in Physcomitrella patens, a model plant among mosses. In this study, the presence of JA was detected in P. patens. The recombinant AOS of P. patens, which was overexpressed in Escherichia coli, showed AOS activity. These data suggest that the octadecanoid pathway also exists in P. patens.

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