The Implication of YggT of Escherichia coli in Osmotic Regulation

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Author(s)

    • Ito Tomokazu ITO Tomokazu
    • Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
    • TAKAYAMA Sayuri
    • Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
    • MATSUDA Nobuyuki
    • Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
    • AIBA Hirofumi
    • Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
    • HEMMI Hisashi
    • Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
    • YOSHIMURA Tohru
    • Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University

Abstract

An <I>Escherichia coli</I> mutant lacking three major K<SUP>+</SUP> uptake systems, Trk, Kup, and Kdp, did not grow under low K<SUP>+</SUP>and high Na<SUP>+</SUP> concentrations. The introduction of <I>fkuA</I> and of <I>fkuB</I> of a marine bacterium, <I>Vibrio alginolyticus</I>, has been reported to compensate for the growth defect by accelerating the rate of K<SUP>+</SUP> uptake (Nakamura, Katoh, Shimizu, Matsuba, and Unemoto, <I>Biochim. Biophys. Acta</I>, <B>1277</B>, 201–208 (1996)). We investigated the function of unknown genes of <I>E. coli</I>, <I>yggS</I> and <I>yggT</I>, homologs of <I>fkuA</I> and <I>fkuB</I> respectively. <I>E. coli</I> TK2420 cells, which lack the three K<SUP>+</SUP> uptake systems, did not grow under high Na<SUP>+</SUP> or mannitol concentrations. The growth defect was compensated by the introduction of the <I>yggT</I> gene alone: <I>yggS</I> was not required. Here we found that YggT endowed <I>E. coli</I> cells with a tolerance for osmotic shock, and discuss a possible mechanism.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 73(12), 2698-2704, 2009-12-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  20

Codes

  • NII Article ID (NAID)
    10027549163
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    10494952
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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