Extensive Mutational Analysis of Modular-Iterative Mixed Polyketide Biosynthesis of Lankacidin in<i>Streptomyces rochei</i>
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- TATSUNO Satoshi
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- ARAKAWA Kenji
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- KINASHI Haruyasu
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
書誌事項
- タイトル別名
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- Extensive Mutational Analysis of Modular-Iterative Mixed Polyketide Biosynthesis of Lankacidin in Streptomyces rochei
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Extensive mutations of lankacidin synthase genes were carried out to analyze the modular-iterative mixed polyketide biosynthesis of lankacidin. Three ketoreductase domains (lkcC-KR, lkcF-KR1, and lkcF-KR2) were inactivated by in-frame deletion and site-directed mutagenesis of their active sites. The mutants ceased or diminished lankacidin production, indicating that the three KR domains are functional in lankacidin biosynthesis. However, all of the KR mutants failed to accumulate the expected unreduced metabolites. Mutational analysis of two tandemly aligned acyl carrier protein domains (lkcC-ACP1 and lkcC-ACP2) revealed that either ACP is sufficient for lankacidin production. Disruption and complementation experiments on three unique genes/domain (lkcD for acyltransferase, lkcB for dehydratase, and lkcC-MT for a C-methyltransferase domain) suggested that their gene products function iteratively during lankacidin biosynthesis.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 73 (12), 2712-2719, 2009
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206477457664
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- NII論文ID
- 10027549212
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10494996
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可